gap43 (Novus Biologicals)
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gap43
Gap43, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+gap+43/GAP-43+Antibody/pm41885803-142-63-65
Average 94 stars, based on 1 article reviews
Gap43, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+gap+43/GAP-43+Antibody/pm41885803-142-63-65
Average 94 stars, based on 1 article reviews
gap43 - by Bioz Stars,
2026-09
94/100 stars
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Incubation:Article Title: Proteostasis and lysosomal repair deficits in transdifferentiated neurons of Alzheimer’s disease Article Snippet: .. The tNeurons were permeabilized with 0.3% Triton X-100 in PBS for 5 min at room temperature or cold methanol on ice for 20 min and blocked with pre-warmed 5% BSA in PBS at room temperature for 45 min. Next, the tNeurons were incubated overnight in a moisture chamber at 4 °C with the following primary antibodies in 5% BSA: anti-ASC/PYCARD (1:100; Santa Cruz, sc-514414), Article Title: Interleukin-4 receptor signaling modulates neuronal network activity Article Snippet: For co-IP, performed as previously described , synaptosome lysates were incubated with anti–IL-4α (BD Bioscience) on protein-A/G agarose beads overnight at 4°C in IP buffer (20 mM Tris-HCL pH 7.5, 150 mM NaCl, 5 mM EDTA, 1 μg/ml pepstatin; Sigma-Aldrich). .. Precipitation was performed by centrifugation with descending speeds (6,000–3,000 g ) for 2 min. Blots were incubated with anti–β-actin (Sigma-Aldrich), anti-PKCγ (Santa Cruz), and Article Title: Proteostasis and lysosomal repair deficits in transdifferentiated neurons of Alzheimer's disease. Article Snippet: .. The tNeurons were permeabilized with 0.3% Triton X-100 in PBS for 5 min at room temperature or cold methanol on ice for 20 min and blocked with pre-warmed 5% BSA in PBS at room temperature for 45 min. Next, the tNeurons were incubated overnight in a moisture chamber at 4 °C with the following primary antibodies in 5% BSA: anti-ASC/PYCARD Nature Cell Biology (1:100; Santa Cruz, sc-514414), Article Title: Proteostasis and lysosomal quality control deficits in Alzheimer’s disease neurons Article Snippet: .. Fibroblasts were incubated with primary antibody solution in 5% BSA overnight at 4°C in a moisture chamber: anti-ASC/PYCARD (1:100; Santa Cruz, sc-514414), Centrifugation:Article Title: Interleukin-4 receptor signaling modulates neuronal network activity Article Snippet: For co-IP, performed as previously described , synaptosome lysates were incubated with anti–IL-4α (BD Bioscience) on protein-A/G agarose beads overnight at 4°C in IP buffer (20 mM Tris-HCL pH 7.5, 150 mM NaCl, 5 mM EDTA, 1 μg/ml pepstatin; Sigma-Aldrich). .. Precipitation was performed by centrifugation with descending speeds (6,000–3,000 g ) for 2 min. Blots were incubated with anti–β-actin (Sigma-Aldrich), anti-PKCγ (Santa Cruz), and Saline:Article Title: The IL ‐4– IL ‐ 4Rα axis modulates olfactory neuroimmune signaling to induce loss of smell Article Snippet: .. Sections were permeabilized using 0.1% Triton in tris‐buffered saline for 5 min followed by antigen retrieval with Tris‐EDTA buffer (pH 9.0) via steamer for 10 min. To avoid nonspecific antibody binding, tissue sections were protein blocked for 30 min. Tissue sections were immunofluorescence (IF) stained with anti‐OMP (Wako, 019‐22291, 1:1000) or Article Title: The IL-4-IL-4Rα axis modulates olfactory neuroimmune signaling to induce loss of smell. Article Snippet: The tissue was then embedded in Surgipath FSC 22 Frozen Section Embedding Medium for sectioning using Leica CM1850, and 20- μm frozen sections were processed from cribriform plate side as described previously.31 For immunofluorescence staining, frozen sections were warmed to room temperature and treated with 10% neutral buffered formalin (NBF) for 10 min. .. Sections were permeabilized using 0.1% Triton in tris- buffered saline for 5 min followed by antigen retrieval with Tris- EDTA buffer (pH 9.0) via steamer for 10 min. To avoid nonspecific antibody binding, tissue sections were protein blocked for 30 min. Tissue sections were immunofluorescence (IF) stained with anti- OMP (Wako, 019- 22291, 1:1000) or Binding Assay:Article Title: The IL ‐4– IL ‐ 4Rα axis modulates olfactory neuroimmune signaling to induce loss of smell Article Snippet: .. Sections were permeabilized using 0.1% Triton in tris‐buffered saline for 5 min followed by antigen retrieval with Tris‐EDTA buffer (pH 9.0) via steamer for 10 min. To avoid nonspecific antibody binding, tissue sections were protein blocked for 30 min. Tissue sections were immunofluorescence (IF) stained with anti‐OMP (Wako, 019‐22291, 1:1000) or Article Title: The IL-4-IL-4Rα axis modulates olfactory neuroimmune signaling to induce loss of smell. Article Snippet: The tissue was then embedded in Surgipath FSC 22 Frozen Section Embedding Medium for sectioning using Leica CM1850, and 20- μm frozen sections were processed from cribriform plate side as described previously.31 For immunofluorescence staining, frozen sections were warmed to room temperature and treated with 10% neutral buffered formalin (NBF) for 10 min. .. Sections were permeabilized using 0.1% Triton in tris- buffered saline for 5 min followed by antigen retrieval with Tris- EDTA buffer (pH 9.0) via steamer for 10 min. To avoid nonspecific antibody binding, tissue sections were protein blocked for 30 min. Tissue sections were immunofluorescence (IF) stained with anti- OMP (Wako, 019- 22291, 1:1000) or Immunofluorescence:Article Title: The IL ‐4– IL ‐ 4Rα axis modulates olfactory neuroimmune signaling to induce loss of smell Article Snippet: .. Sections were permeabilized using 0.1% Triton in tris‐buffered saline for 5 min followed by antigen retrieval with Tris‐EDTA buffer (pH 9.0) via steamer for 10 min. To avoid nonspecific antibody binding, tissue sections were protein blocked for 30 min. Tissue sections were immunofluorescence (IF) stained with anti‐OMP (Wako, 019‐22291, 1:1000) or Article Title: The IL-4-IL-4Rα axis modulates olfactory neuroimmune signaling to induce loss of smell. Article Snippet: The tissue was then embedded in Surgipath FSC 22 Frozen Section Embedding Medium for sectioning using Leica CM1850, and 20- μm frozen sections were processed from cribriform plate side as described previously.31 For immunofluorescence staining, frozen sections were warmed to room temperature and treated with 10% neutral buffered formalin (NBF) for 10 min. .. Sections were permeabilized using 0.1% Triton in tris- buffered saline for 5 min followed by antigen retrieval with Tris- EDTA buffer (pH 9.0) via steamer for 10 min. To avoid nonspecific antibody binding, tissue sections were protein blocked for 30 min. Tissue sections were immunofluorescence (IF) stained with anti- OMP (Wako, 019- 22291, 1:1000) or Staining:Article Title: The IL ‐4– IL ‐ 4Rα axis modulates olfactory neuroimmune signaling to induce loss of smell Article Snippet: .. Sections were permeabilized using 0.1% Triton in tris‐buffered saline for 5 min followed by antigen retrieval with Tris‐EDTA buffer (pH 9.0) via steamer for 10 min. To avoid nonspecific antibody binding, tissue sections were protein blocked for 30 min. Tissue sections were immunofluorescence (IF) stained with anti‐OMP (Wako, 019‐22291, 1:1000) or Article Title: The IL-4-IL-4Rα axis modulates olfactory neuroimmune signaling to induce loss of smell. Article Snippet: The tissue was then embedded in Surgipath FSC 22 Frozen Section Embedding Medium for sectioning using Leica CM1850, and 20- μm frozen sections were processed from cribriform plate side as described previously.31 For immunofluorescence staining, frozen sections were warmed to room temperature and treated with 10% neutral buffered formalin (NBF) for 10 min. .. Sections were permeabilized using 0.1% Triton in tris- buffered saline for 5 min followed by antigen retrieval with Tris- EDTA buffer (pH 9.0) via steamer for 10 min. To avoid nonspecific antibody binding, tissue sections were protein blocked for 30 min. Tissue sections were immunofluorescence (IF) stained with anti- OMP (Wako, 019- 22291, 1:1000) or |